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Title:

Genome-wide transposon screening and quantitative insertion site sequencing for cancer gene discovery in mice.

Document type:
Journal Article; 2
Author(s):
Friedrich, Mathias J; Rad, Lena; Bronner, Iraad F; Strong, Alexander; Wang, Wei; Weber, Julia; Mayho, Matthew; Ponstingl, Hannes; Engleitner, Thomas; Grove, Carolyn; Pfaus, Anja; Saur, Dieter; Cadiñanos, Juan; Quail, Michael A; Vassiliou, George S; Liu, Pentao; Bradley, Allan; Rad, Roland
Abstract:
Transposon-mediated forward genetics screening in mice has emerged as a powerful tool for cancer gene discovery. It pinpoints cancer drivers that are difficult to find with other approaches, thus complementing the sequencing-based census of human cancer genes. We describe here a large series of mouse lines for insertional mutagenesis that are compatible with two transposon systems, PiggyBac and Sleeping Beauty, and give guidance on the use of different engineered transposon variants for constitu...     »
Journal title abbreviation:
Nat Protoc
Year:
2017
Journal volume:
12
Journal issue:
2
Pages contribution:
289-309
Fulltext / DOI:
doi:10.1038/nprot.2016.164
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/28079877
Print-ISSN:
1754-2189
TUM Institution:
Professur für Translationale gastroenterologische Onkologie (Prof. Rad)
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