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Titel:

EASY-HIT: HIV full-replication technology for broad discovery of multiple classes of HIV inhibitors.

Dokumenttyp:
Journal Article; Article
Autor(en):
Kremb, S; Helfer, M; Heller, W; Hoffmann, D; Wolff, H; Kleinschmidt, A; Cepok, S; Hemmer, B; Durner, J; Brack-Werner, R
Abstract:
HIV replication assays are important tools for HIV drug discovery efforts. Here, we present a full HIV replication system (EASY-HIT) for the identification and analysis of HIV inhibitors. This technology is based on adherently growing HIV-susceptible cells, with a stable fluorescent reporter gene activated by HIV Tat and Rev. A fluorescence-based assay was designed that measures HIV infection by two parameters relating to the early and the late phases of HIV replication, respectively. Validation...     »
Zeitschriftentitel:
Antimicrob Agents Chemother
Jahr:
2010
Band / Volume:
54
Heft / Issue:
12
Seitenangaben Beitrag:
5257-68
Sprache:
eng
Volltext / DOI:
doi:10.1128/AAC.00515-10
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/20876377
Print-ISSN:
0066-4804
TUM Einrichtung:
Institut für Virologie ; Neurologische Klinik und Poliklinik
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