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Titel:

Protein A Affinity Precipitation of Human Immunoglobulin G

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
L. Janoschek; M. Freiherr von Roman; S. Berensmeier
Abstract:
The potential of protein A affinity precipitation as an alternative method for traditional antibody purification techniques was investigated. Recombinant produced protein A from Staphylococcus aureus (SpA) was covalently linked to the pH-responsive copolymer Eudragit® S-100 and used for purification of human immunoglobulin G (hIgG). The Eudragit-SpA conjugate had a static binding capacity of 93.9 ± 2.8 mg hIgG per g conjugate and a dissociation constant of 787 ± 67 nM at 7 ± 1 °C. The antibody w...     »
Stichworte:
Antibody purification; Eudragit; Affinity precipitation; protein A; Immunoglobulin G
Zeitschriftentitel:
Journal of Chromatography B
Jahr:
2014
Nachgewiesen in:
Web of Science
Reviewed:
ja
Sprache:
en
Volltext / DOI:
doi:10.1016/j.jchromb.2014.06.011
WWW:
http://authors.elsevier.com/a/1PJ0t_Zihpc99L
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