Novel indirect quality parameters were established to predict the baking performance of wheat flours. In particular the concentrations of gliadins (GLIA), glutenins (GLUT), and glutenin macropolymer (GMP) were suitable because they were highly correlated with the bread volumes determined by a micro baking test (MBT). Furthermore, simple and easy-to-use methods based on colorimetry and fluorimetry for the quantitation of GLIA-, GLUT-, and GMP-concentrations were developed. Among these, the naphthol blue black (NBB) assay showed the best results and has the potential to be applied as a simple high throughput method for determining technologically relevant protein parameters of wheat flours.
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Novel indirect quality parameters were established to predict the baking performance of wheat flours. In particular the concentrations of gliadins (GLIA), glutenins (GLUT), and glutenin macropolymer (GMP) were suitable because they were highly correlated with the bread volumes determined by a micro baking test (MBT). Furthermore, simple and easy-to-use methods based on colorimetry and fluorimetry for the quantitation of GLIA-, GLUT-, and GMP-concentrations were developed. Among these, the naphth...
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