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Title:

Fractionation of α-lactalbumin and β-lactoglobulin from whey protein isolate using selective thermal aggregation, an optimized membrane separation procedure and resolubilization techniques at pilot plant scale

Document type:
Zeitschriftenaufsatz
Author(s):
Toro-Sierra, J.; Tolkach, A.; Kulozik, U.
Abstract:
An optimized fractionation method in the pilot scale for production of isolated α-lactalbumin (α-La) and βlactoglobulin (β-Lg) was developed. The method comprises following steps: (1) selective thermal precipitation of α-La, (2) aging of the formed particles, (3) separation of native β-Lg from the precipitate via microfiltration and ultrafiltration, (4) purification of β-Lg, (5) resolubilization of the precipitate, and (6) purification of α-La. The native status of the isolated fractions was con...     »
Keywords:
alpha-Lactalbumin; Diafiltration; Membrane filtration; ß-Lactoglobulin; Whey proteins
Journal title:
Food and Bioprocess Technology
Year:
2013
Journal volume:
6
Journal issue:
4
Pages contribution:
1032--1043
Fulltext / DOI:
doi:10.1007/s11947-011-0732-2
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