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Title:

Chymotrypsin selectively digests β-lactoglobulin in whey protein isolate away from enzyme optimal conditions: potential for native α-lactalbumin purification

Document type:
Zeitschriftenaufsatz
Author(s):
Lisak, K.; Toro-Sierra, J.; Kulozik, U.; Božanić, R.; Cheison, S. C.
Abstract:
The present study examines the resistance of the α-lactalbumin to α-chymotrypsin (EC 3.4.21.1) digestion under various experimental conditions. Whey protein isolate (WPI) was hydrolysed using randomised hydrolysis conditions (5 and 10% of WPI; pH 7.0, 7.8 and 8.5; temperature 25, 37 and 50 °C; enzyme-to-substrate ratio, E/S, of 0.1%, 0.5 and 1%). Reversed-phase high performance liquid chromatography (RP-HPLC) was used to analyse residual proteins. Heat, pH adjustment and two inhibitors (Bowma...     »
Keywords:
Animals; Chromatography, High Pressure Liquid; Chymotrypsin/metabolism; Hot Temperature; Hydrogen-Ion Concentration; hydrolysis; Lactalbumin/isolation {&} purification; Lactoglobulins/metabolism; Milk; Milk Proteins/chemistry; Protease Inhibitors/pharmacology; Substrate Specificity; Whey proteins
Journal title:
The Journal of Dairy Research
Year:
2013
Journal volume:
80
Journal issue:
1
Pages contribution:
14--20
Fulltext / DOI:
doi:10.1017/S0022029912000416
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