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Title:

High pressure stability of protein complexes studied by static and dynamic light scattering

Document type:
Zeitschriftenaufsatz
Author(s):
Gebhardt, R.; Kulozik, U.
Abstract:
The high pressure dissociation of hemocyanin prepared from the lobster Homarus americanus and casein micelles from cow milk were observed by in situ light scattering. The hemocyanin dodecamer dissociated via a hexamer into monomers in a two-step three-species reaction. The influence of ligands and the effector l-lactate on the dissociation behaviorwas investigated. While no effect by carbon monoxide after exchanging the ligand oxygen was observed, the addition of the effector l-lactate led to a...     »
Keywords:
Intermolecular interactions; Ligand binding; Protein complexes; Static and dynamic light scattering
Journal title:
High Pressure Research
Year:
2011
Journal volume:
31
Journal issue:
1
Pages contribution:
243--252
Fulltext / DOI:
doi:10.1080/08957959.2010.543900
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