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- Original title:
- Charakterisierung der Enzymausstattung von Clostridium stercorarium für den vollständigen Abbau von Hemicellulosen
- Translated title:
- Characterisation of the Clostridium stercorarium enzymes for the complete degradation of hemicelluloses
- Author:
- Bröker, Jannis
- Year:
- 2019
- Document type:
- Dissertation
- Faculty/School:
- Fakultät Wissenschaftszentrum Weihenstephan
- Advisor:
- Liebl, Wolfgang (Prof. Dr.)
- Referee:
- Liebl, Wolfgang (Prof. Dr.); Vogel, Rudi F. (Prof. Dr.)
- Language:
- de
- Subject group:
- BIO Biowissenschaften
- Keywords:
- Clostridium stercorarium, Biomasseabbau, Enzymcharakterisierung, Hemicellulose, Arabinoxylan, Glycosidhydrolasen, Xylanasen, Arabinofuranosidasen, Enzymkombination, Produktspezifität, Thermostabilität, Produktinhibition, Kohlenhydratesterasen
- Translated keywords:
- C. stercorarium, Biomass degradation, Enzyme characterisation, Hemicellulose, Arabinoxylan, Glycosid Hydrolases, Xylanases, Arabinofuranosidases, Enzyme combination, Product specificity, Thermostability, Product inhibition, Carbohydrate Esterases
- TUM classification:
- BIO 250d
- Abstract:
- Clostridium stercorarium ist ein thermophiles, saccharolytisches Bakterium, das hervorragend an den Abbau von Hemicellulose angepasst ist. Um das hemicellulolytische Potential von C. stercorarium und seiner thermostabilen Enzyme zu ermitteln, wurden 50 Glycosidhydrolasen und 8 Kohlenhydratesterasen heterolog in Escherichia coli produziert und charakterisiert. Die rekombinanten Enzyme zeigten einzeln und in Kombination Aktivität für Xylan, β-Glucan, Xyloglucan, Mannane, Arabinan und Galactan.
- Translated abstract:
- Clostridium stercorarium is a thermophilic, saccharolytic bacterium which is well adapted to the degradation of hemicellulose. To reveal the hemicellulolytic potential of C. stercorarium and its thermostable enzymes, 50 glycoside hydrolases and 8 carbohydrate esterases were heterologously produced in Escherichia coli and characterised. The recombinant enzymes showed activity individually and in combination for xylan, β-glucan, xyloglucan, mannans, arabinan and galactan.
- WWW:
- https://mediatum.ub.tum.de/?id=1506541
- Date of submission:
- 01.07.2019
- Oral examination:
- 25.11.2019
- File size:
- 4708390 bytes
- Pages:
- 176
- Urn (citeable URL):
- https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20191125-1506541-1-5
- Last change:
- 25.11.2020
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