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Title:

Generation of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems.

Document type:
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Kugler, Franziska; Drexler, Ingo; Protzer, Ulrike; Hoffmann, Dieter; Moeini, Hassan
Abstract:
BACKGROUND: Recombinant Modified Vaccinia Virus Ankara has been employed as a safe and potent viral vector vaccine against infectious diseases and cancer. We generated recMVAs encoding norovirus GII.4 genotype capsid protein by using a marker-based approach and a BAC-based system. In the marker-based approach, the capsid gene together with a reporter gene was introduced into the MVA genome in DF-1 cells. Several rounds of plaque purification were carried out to get rid of the WT-MVA. In the BAC-...     »
Journal title abbreviation:
Virol J
Year:
2019
Journal volume:
16
Journal issue:
1
Fulltext / DOI:
doi:10.1186/s12985-019-1212-y
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/31399106
Print-ISSN:
1743-422X
TUM Institution:
Institut für Virologie
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