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Titel:

Generation of recombinant MVA-norovirus: a comparison study of bacterial artificial chromosome- and marker-based systems.

Dokumenttyp:
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Kugler, Franziska; Drexler, Ingo; Protzer, Ulrike; Hoffmann, Dieter; Moeini, Hassan
Abstract:
BACKGROUND: Recombinant Modified Vaccinia Virus Ankara has been employed as a safe and potent viral vector vaccine against infectious diseases and cancer. We generated recMVAs encoding norovirus GII.4 genotype capsid protein by using a marker-based approach and a BAC-based system. In the marker-based approach, the capsid gene together with a reporter gene was introduced into the MVA genome in DF-1 cells. Several rounds of plaque purification were carried out to get rid of the WT-MVA. In the BAC-...     »
Zeitschriftentitel:
Virol J
Jahr:
2019
Band / Volume:
16
Heft / Issue:
1
Volltext / DOI:
doi:10.1186/s12985-019-1212-y
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/31399106
Print-ISSN:
1743-422X
TUM Einrichtung:
Institut für Virologie
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