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Title:

Isolation and Culture of Oligodendrocytes.

Document type:
Journal Article
Author(s):
Weil, Marie-Theres; Schulz-Ëberlin, Giselheid; Mukherjee, Chaitali; Kuo-Elsner, Wen Ping; Schäfer, Isabelle; Müller, Christina; Simons, Mikael
Abstract:
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and mye...     »
Journal title abbreviation:
Methods Mol Biol
Year:
2019
Journal volume:
1936
Pages contribution:
79-95
Fulltext / DOI:
doi:10.1007/978-1-4939-9072-6_5
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/30820894
Print-ISSN:
1064-3745
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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