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Titel:

Isolation and Culture of Oligodendrocytes.

Dokumenttyp:
Journal Article
Autor(en):
Weil, Marie-Theres; Schulz-Ëberlin, Giselheid; Mukherjee, Chaitali; Kuo-Elsner, Wen Ping; Schäfer, Isabelle; Müller, Christina; Simons, Mikael
Abstract:
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and mye...     »
Zeitschriftentitel:
Methods Mol Biol
Jahr:
2019
Band / Volume:
1936
Seitenangaben Beitrag:
79-95
Volltext / DOI:
doi:10.1007/978-1-4939-9072-6_5
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/30820894
Print-ISSN:
1064-3745
TUM Einrichtung:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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