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Title:

Lysate Preparation for Reverse Phase Protein Arrays.

Document type:
Journal Article; Review
Author(s):
Becker, Karl-Friedrich
Abstract:
Protein lysates from a variety of sample materials, e.g., cell lines, serum, frozen tissues, formalin-fixed and paraffin-embedded (FFPE) tissues, and fine needle aspirates, are compatible with the analysis using reverse phase protein arrays (RPPAs). Due to this diversity of input material, lysate preparation is one of the critical steps for obtaining reliable RPPA results. The challenges include, but are not limited to, achieving complete solubilization, avoiding chemical or enzymatic protein mo...     »
Journal title abbreviation:
Adv Exp Med Biol
Year:
2019
Journal volume:
1188
Pages contribution:
21-30
Fulltext / DOI:
doi:10.1007/978-981-32-9755-5_2
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/31820381
Print-ISSN:
0065-2598
TUM Institution:
Institut für Allgemeine Pathologie und Pathologische Anatomie
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