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Title:

Efficient immunoaffinity chromatography of lymphocytes directly from whole blood.

Document type:
Journal Article
Author(s):
Mohr, Fabian; Przibilla, Sabine; Leonhardt, Franziska; Stemberger, Christian; Dreher, Stefan; Müller, Thomas R; Fräßle, Simon P; Schmidt, Georg P; Kiene, Marie-Luise; Stadler, Herbert; Busch, Dirk H
Abstract:
We show that defined lymphocytes can be rapidly purified by immunoaffinity chromatography starting directly from whole blood. The method relies on low-affinity Fab-fragments attached to a column-matrix combined with the reversible Strep-tag technology. Compared to established cell enrichment protocols, the Strep-tag affinity chromatography of cells is independent of erythrocyte lysis or centrifugation steps, allowing for simple cell-enrichment with good yields, high purities, and excellent funct...     »
Journal title abbreviation:
Sci Rep
Year:
2018
Journal volume:
8
Journal issue:
1
Fulltext / DOI:
doi:10.1038/s41598-018-34589-z
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/30425259
Print-ISSN:
2045-2322
TUM Institution:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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