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Document type:
Journal Article; Research Support, Non-U.S. Gov't 
Author(s):
Pavlovic, Melanie; Koehler, Nina; Anton, Martina; Dinkelmeier, Anna; Haase, Maren; Stellberger, Thorsten; Busch, Ulrich; Baiker, Armin E 
Title:
Reverse Transcription Quantitative Polymerase Chain Reaction for Detection of and Differentiation Between RNA and DNA of HIV-1-Based Lentiviral Vectors. 
Abstract:
The purpose of the described method is the detection of and differentiation between RNA and DNA of human immunodeficiency virus (HIV)-derived lentiviral vectors (LV) in cell culture supernatants and swab samples. For the analytical surveillance of genetic engineering, operations methods for the detection of the HIV-1-based LV generations are required. Furthermore, for research issues, it is important to prove the absence of LV particles for downgrading experimental settings in terms of the biosa...    »
 
Journal title abbreviation:
Hum Gene Ther Methods 
Year:
2017 
Journal volume:
28 
Journal issue:
Pages contribution:
215-221 
Language:
eng 
Fulltext / DOI:
Print-ISSN:
1946-6536 
TUM Institution:
Institut für Experimentelle Onkologie und Therapieforschung