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Document type:
Journal Article; Article 
Author(s):
Berninger, Markus T; Mohajerani, Pouyan; Kimm, Melanie; Masius, Stephan; Ma, Xiaopeng; Wildgruber, Moritz; Haller, Bernhard; Anton, Martina; Imhoff, Andreas B; Ntziachristos, Vasilis; Henning, Tobias D; Meier, Reinhard 
Title:
Fluorescence molecular tomography of DiR-labeled mesenchymal stem cell implants for osteochondral defect repair in rabbit knees. 
Abstract:
To assess labelling efficiency of rabbit mesenchymal stem cells (MSCs) using the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) and detection of labelled MSCs for osteochondral defect repair in a rabbit model using fluorescence molecular tomography-X-ray computed tomography (FMT-XCT).MSCs were isolated from New Zealand White rabbits and labelled with DiR (1.25-20 ?g/mL). Viability and induction of apoptosis were assessed by XTT- and Caspase-3/-7-testing. Chondrogenic potential was evaluated by measurement of glycosaminoglycans. Labelled cells and unlabeled controls (n = 3) underwent FMT-XCT imaging before and after chondrogenic differentiation. Osteochondral defects were created surgically in rabbit knees (n = 6). Unlabeled and labelled MSCs were implanted in fibrin-clots and imaged by FMT-XCT. Statistical analyses were performed using multiple regression models.DiR-labelling of MSCs resulted in a dose-dependent fluorescence signal on planar images in trans-illumination mode. No significant reduction in viability or induction of apoptosis was detected at concentrations below 10 ?g DiR/mL (p > .05); the chondrogenic potential of MSCs was not affected (p > .05). FMT-XCT of labelled MSCs in osteochondral defects showed a significant signal of the transplant (p < .05) with additional high-resolution anatomical information about its osteochondral integration.FMT-XCT allows for detection of stem cell implantation within osteochondral regeneration processes.o DiR-labelling of MSCs shows no toxic side effects or impairment of chondrogenesis. o Fluorescence molecular tomography allows for detection of MSCs for osteochondral defect repair. o FMT-XCT helps to improve evaluation of cell implantation and osteochondral regeneration processes. 
Journal title abbreviation:
Eur Radiol 
Year:
2017 
Journal volume:
27 
Journal issue:
Pages contribution:
1105-1113 
Language:
eng 
Print-ISSN:
0938-7994 
TUM Institution:
Fachgebiet Sportorthopädie (Prof. Imhoff); Institut für Experimentelle Onkologie und Therapieforschung; Institut für Medizinische Statistik und Epidemiologie; Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)