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Titel:

Immobilization of soluble protein complexes in MAS solid-state NMR: Sedimentation versus viscosity

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Sarkar, Riddhiman; Mainz, Andi; Busi, Baptiste; Barbet-Massin, Emeline; Kranz, Maximilian; Hofmann, Thomas; Reif, Bernd
Abstract:
In recent years, MAS solid-state NMR has emerged as a technique for the investigation of soluble protein complexes. It was found that high molecular weight complexes do not need to be crystallized in order to obtain an immobilized sample for solid-state NMR investigations. Sedimentation induced by sample rotation impairs rotational diffusion of proteins and enables efficient dipolar coupling based cross polarization transfers. In addition, viscosity contributes to the immobilization of the molec...     »
Zeitschriftentitel:
Solid state nuclear magnetic resonance
Jahr:
2016
Band / Volume:
76-77
Jahr / Monat:
2016-03
Seitenangaben Beitrag:
7--14
Volltext / DOI:
doi:10.1016/j.ssnmr.2016.03.005
Print-ISSN:
0926-2040
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