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Title:

Establishment of a 3D cell culture model of primary bovine mammary epithelial cells extracted from fresh milk

Document type:
Zeitschriftenaufsatz
Author(s):
Hillreiner, Maria; Müller, Nadine I.; Koch, Heiner M.; Schmautz, Christiane; Küster, Bernhard; Pfaffl, Michael W.; Kliem, Heike
Abstract:
For the investigation of molecular processes underlying diseases of the bovine mammary gland, primary bovine mammary epithelial cells (pbMEC) are used. They are known to contribute to the innate immune system of the bovine mammary gland. The functionality of pbMEC depends on the maintenance of in vivo characteristics. So far, the optimization of pbMEC culture conditions was intended in a variety of experiments. For this purpose, most of the studies used stable cell lines or primary cells obtaine...     »
Keywords:
3D cell culture; Extracellular matrix; Lactogenesis; Primary bovine mammary epithelial cells; Proteomics; RT-qPCR
Journal title:
In Vitro Cellular & Developmental Biology - Animal
Year:
2017
Fulltext / DOI:
doi:10.1007/s11626-017-0169-7
Publisher:
Springer Nature
E-ISSN:
1071-26901543-706X
Date of publication:
22.06.2017
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