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Title:

Expression and Purification of EPHA2 Tyrosine Kinase Domain for Crystallographic and NMR Studies

Document type:
Zeitschriftenaufsatz
Author(s):
Gande, Santosh L.; Saxena, Krishna; Sreeramulu, Sridhar; Linhard, Verena; Kudlinzki, Denis; Heinzlmeir, Stephanie; Reichert, Andreas J.; Skerra, Arne; Kuster, Bernhard; Schwalbe, Harald
Abstract:
The receptor tyrosine kinase EPHA2 is overexpressed in several cancer entities (breast, head and neck, non-small cell lung cancer). Small molecule-based inhibition of the activity of the EPHA2 kinase domain (KD) has been seen as an important strategy for achieving therapeutic intervention. However, obtaining structural information by protein crystallography or by NMR spectroscopy aiding drug discovery, has been severely hampered due to the lack of pure and homogeneous protein. Here different fra...     »
Keywords:
BayBioMS
Journal title:
ChemBioChem
Year:
2016
Journal volume:
17
Journal issue:
23
Pages contribution:
2257-2263
Fulltext / DOI:
doi:10.1002/cbic.201600483
Publisher:
Wiley-Blackwell
E-ISSN:
1439-4227
Date of publication:
09.11.2016
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