Benutzer: Gast  Login
Titel:

Trimodal Mixed Mode Chromatography That Enables Efficient Offline Two-Dimensional Peptide Fractionation for Proteome Analysis

Dokumenttyp:
Zeitschriftenaufsatz
Autor(en):
Yu, Peng; Petzoldt, Svenja; Wilhelm, Mathias; Zolg, Daniel Paul; Zheng, Runsheng; Sun, Xuefei; Liu, Xiaodong; Schneider, Günter; Huhmer, Andreas; Kuster, Bernhard
Abstract:
Offline two-dimensional chromatography is a common means to achieve deep proteome coverage. To reduce sample complexity and dynamic range and to utilize mass spectrometer (MS) time efficiently, high chromatographic resolution of and good orthogonality between the two dimensions are needed. Ion exchange and high pH reversed phase chromatography are often used for this purpose. However, the former requires desalting to be MS-compatible, and the latter requires fraction pooling to create orthogonal...     »
Stichworte:
BayBioMS; Protein identificationPeptide identificationPeptides and proteinsIon exchangeChromatography
Zeitschriftentitel:
Analytical Chemistry
Jahr:
2017
Band / Volume:
89
Heft / Issue:
17
Seitenangaben Beitrag:
8884-8891
Volltext / DOI:
doi:10.1021/acs.analchem.7b01356
Verlag / Institution:
American Chemical Society (ACS)
E-ISSN:
0003-27001520-6882
Publikationsdatum:
18.08.2017
 BibTeX