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Title:

Near-infrared branding efficiently correlates light and electron microscopy.

Document type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
Author(s):
Bishop, Derron; Niki?, Ivana; Brinkoetter, Mary; Knecht, Sharmon; Potz, Stephanie; Kerschensteiner, Martin; Misgeld, Thomas
Abstract:
The correlation of light and electron microscopy of complex tissues remains a major challenge. Here we report near-infrared branding (NIRB), which facilitates such correlation by using a pulsed, near-infrared laser to create defined fiducial marks in three dimensions in fixed tissue. As these marks are fluorescent and can be photo-oxidized to generate electron contrast, they can guide re-identification of previously imaged structures as small as dendritic spines by electron microscopy.
Journal title abbreviation:
Nat Methods
Year:
2011
Journal volume:
8
Journal issue:
7
Pages contribution:
568-70
Language:
eng
Fulltext / DOI:
doi:10.1038/nmeth.1622
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/21642966
Print-ISSN:
1548-7091
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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