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Titel:

A sensitive method for determining the phosphorylation status of natriuretic peptide receptors: cGK-Ialpha does not regulate NPR-A.

Dokumenttyp:
Comparative Study; Journal Article; Research Support, N.I.H., Extramural
Autor(en):
Bryan, PM; Smirnov, D; Smolenski, A; Feil, S; Feil, R; Hofmann, F; Lohmann, S; Potter, LR
Abstract:
Natriuretic peptide receptor A (NPR-A) and natriuretic peptide receptor B (NPR-B) are transmembrane guanylyl cyclases that catalyze the synthesis of cGMP in response to natriuretic peptides. Phosphorylation and dephosphorylation regulate these receptors and have been traditionally studied by (32)PO(4) labeling of transfected cells. However, this approach cannot be used to determine the phosphorylation state of receptors isolated from unlabeled sources. Here, we use Pro-Q Diamond and SYPRO Ruby d...     »
Zeitschriftentitel:
Biochemistry
Jahr:
2006
Band / Volume:
45
Heft / Issue:
4
Seitenangaben Beitrag:
1295-303
Sprache:
eng
Volltext / DOI:
doi:10.1021/bi051253d
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/16430226
Print-ISSN:
0006-2960
TUM Einrichtung:
Institut für Pharmakologie und Toxikologie
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