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Document type:
journal article 
Author(s):
Hoffmann, D; Buchberger, B; Nemetz, C 
Title:
In vitro synthesis of enzymatically active HIV-1 protease for rapid phenotypic resistance profiling. 
Abstract:
BACKGROUND: Given the expanding antiretroviral therapy, inexpensive and fast HIV drug resistance assays are urgently needed. In this view, we have developed a novel phenotypic resistance test for HIV-1 protease inhibitors (PIs) based on recombinant expression of patient-derived HIV PR in Escherichia coli and subsequent enzymatic testing in a fluorescent readout. OBJECTIVES: To facilitate and expedite the test procedure, we have introduced coupled in vitro transcription/translation using a commer...    »
 
Journal title abbreviation:
J Clin Virol 
Year:
2005 
Journal volume:
32 
Journal issue:
Pages contribution:
294-9 
Language:
eng 
Print-ISSN:
1386-6532 
TUM Institution:
Institut für Virologie