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Title:

In vitro synthesis of enzymatically active HIV-1 protease for rapid phenotypic resistance profiling.

Document type:
Journal Article
Author(s):
Hoffmann, D; Buchberger, B; Nemetz, C
Abstract:
BACKGROUND: Given the expanding antiretroviral therapy, inexpensive and fast HIV drug resistance assays are urgently needed. In this view, we have developed a novel phenotypic resistance test for HIV-1 protease inhibitors (PIs) based on recombinant expression of patient-derived HIV PR in Escherichia coli and subsequent enzymatic testing in a fluorescent readout. OBJECTIVES: To facilitate and expedite the test procedure, we have introduced coupled in vitro transcription/translation using a commer...     »
Journal title abbreviation:
J Clin Virol
Year:
2005
Journal volume:
32
Journal issue:
4
Pages contribution:
294-9
Language:
eng
Fulltext / DOI:
doi:10.1016/j.jcv.2004.09.016
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/15780808
Print-ISSN:
1386-6532
TUM Institution:
Institut für Virologie
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