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Title:

Quantitation of MHC tetramer-positive cells from whole blood: evaluation of a single-platform, six-parameter flow cytometric method.

Document type:
Evaluation Studies; Journal Article
Author(s):
Wölfl, M; Schalk, S; Hellmich, M; Huster, KM; Busch, DH; Berthold, F
Abstract:
BACKGROUND: Quantitation of antigen-specific T cells provides an insight into the development and dynamics of T-cell responses in tumor immunology and infectious diseases. Soluble major histocompatibility class I tetramers are widely used to monitor immune responses; however, variations due to handling and analysis are likely to confound comparisons between different experiments and laboratories. METHODS: Whole blood from healthy donors was stained with HLA-A*0201/tetramers specific for an epit...     »
Journal title abbreviation:
Cytometry A
Year:
2004
Journal volume:
57
Journal issue:
2
Pages contribution:
120-30
Language:
eng
Fulltext / DOI:
doi:10.1002/cyto.a.10116
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/14750134
Print-ISSN:
1552-4922
TUM Institution:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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