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Titel:

Quantitation of MHC tetramer-positive cells from whole blood: evaluation of a single-platform, six-parameter flow cytometric method.

Dokumenttyp:
Evaluation Studies; Journal Article
Autor(en):
Wölfl, M; Schalk, S; Hellmich, M; Huster, KM; Busch, DH; Berthold, F
Abstract:
BACKGROUND: Quantitation of antigen-specific T cells provides an insight into the development and dynamics of T-cell responses in tumor immunology and infectious diseases. Soluble major histocompatibility class I tetramers are widely used to monitor immune responses; however, variations due to handling and analysis are likely to confound comparisons between different experiments and laboratories. METHODS: Whole blood from healthy donors was stained with HLA-A*0201/tetramers specific for an epit...     »
Zeitschriftentitel:
Cytometry A
Jahr:
2004
Band / Volume:
57
Heft / Issue:
2
Seitenangaben Beitrag:
120-30
Sprache:
eng
Volltext / DOI:
doi:10.1002/cyto.a.10116
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/14750134
Print-ISSN:
1552-4922
TUM Einrichtung:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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