User: Guest  Login
Title:

Serial induction of mutations by ethylnitrosourea in PC12 cells: a new model for a phenotypical characterization of the neurotoxic response to 6-hydroxydopamine.

Document type:
Journal Article
Author(s):
Schlegel, J; Neff, F; Piontek, G
Abstract:
Here we show that the serial generation of allelic mutations by treatment with the mutagen ethylnitrosourea (ENU) in PC12 cells provides a new model for the phenotypical dissection of biological properties. We tested this approach in the neurotoxic 6-OHDA model of Parkinson's disease in PC12 cells which has been widely used as an in vitro model for the investigation of the molecular pathogenesis of neuronal cell death and for novel treatment approaches. ENU treatment at doses of 0.2 and 0.3 mg/m...     »
Journal title abbreviation:
J Neurosci Methods
Year:
2004
Journal volume:
137
Journal issue:
2
Pages contribution:
215-20
Language:
eng
Fulltext / DOI:
doi:10.1016/j.jneumeth.2004.02.010
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/15262063
Print-ISSN:
0165-0270
TUM Institution:
Institut für Allgemeine Pathologie und Pathologische Anatomie
 BibTeX