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Titel:

Generation of Tissue Sections for Screening Thy1 Mouse Lines.

Dokumenttyp:
Journal Article
Autor(en):
Marinkovi?, Petar; Godinho, Leanne; Misgeld, Thomas
Abstract:
New generations of Thy1-XFP transgenic mice (where XFP stands for any fluorescent protein) can now be readily generated, given the availability of core facilities or commercial providers of Thy1 pronuclear injections. Here, we provide a protocol for screening founder progeny. Transcardial perfusion is performed on 3-wk-old F1 mice that have been produced by crossing Thy1 transgenic founders and commercially obtained inbred mice. Cryosections are generated, and Thy1-driven expression is detected...     »
Zeitschriftentitel:
Cold Spring Harb Protoc
Jahr:
2015
Band / Volume:
2015
Heft / Issue:
9
Seitenangaben Beitrag:
pdb.prot087684
Sprache:
eng
Volltext / DOI:
doi:10.1101/pdb.prot087684
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/26330627
Print-ISSN:
1940-3402
TUM Einrichtung:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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