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Title:

Generation of Thy1 Constructs for Pronuclear Injection.

Document type:
Journal Article
Author(s):
Marinkovi?, Petar; Godinho, Leanne; Misgeld, Thomas
Abstract:
With easy access to core facilities or commercial providers of pronuclear injections, generating simple Thy1-XFP transgenic mice (where XFP stands for any fluorescent protein) is now a possibility even for small laboratories. The generation of new Thy1 transgenic lines generally consists of five steps: (1) engineering and characterization of the desired fluorescent reporter protein, (2) cloning of the reporter protein into the Thy1 vector, (3) linearization and purification of the new Thy1 const...     »
Journal title abbreviation:
Cold Spring Harb Protoc
Year:
2015
Journal volume:
2015
Journal issue:
10
Pages contribution:
937-40
Language:
eng
Fulltext / DOI:
doi:10.1101/pdb.prot087676
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/26430258
Print-ISSN:
1940-3402
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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