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Title:

Cellular, subcellular and functional in vivo labeling of the spinal cord using vital dyes.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Romanelli, Elisa; Sorbara, Catherine D; Niki?, Ivana; Dagkalis, Athanasios; Misgeld, Thomas; Kerschensteiner, Martin
Abstract:
Here we provide a protocol for rapidly labeling different cell types, distinct subcellular compartments and key injury mediators in the spinal cord of living mice. This method is based on the application of synthetic vital dyes to the surgically exposed spinal cord. Suitable vital dyes applied in appropriate concentrations lead to reliable in vivo labeling, which can be combined with genetic tags and in many cases preserved for postfixation analysis. In combination with in vivo imaging, this app...     »
Journal title abbreviation:
Nat Protoc
Year:
2013
Journal volume:
8
Journal issue:
3
Pages contribution:
481-90
Language:
eng
Fulltext / DOI:
doi:10.1038/nprot.2013.022
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/23391891
Print-ISSN:
1754-2189
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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