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Title:

In vivo imaging of mitochondria in intact zebrafish larvae.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Paquet, Dominik; Pluci?ska, Gabriela; Misgeld, Thomas
Abstract:
Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural...     »
Journal title abbreviation:
Methods Enzymol
Year:
2014
Journal volume:
547
Pages contribution:
151-64
Language:
eng
Fulltext / DOI:
doi:10.1016/B978-0-12-801415-8.00009-6
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/25416357
Print-ISSN:
0076-6879
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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