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Title:

A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Buchfellner, Andrea; Yurlova, Larisa; Nüske, Stefan; Scholz, Armin M; Bogner, Jacqueline; Ruf, Benjamin; Zolghadr, Kourosh; Drexler, Sophie E; Drexler, Guido A; Girst, Stefanie; Greubel, Christoph; Reindl, Judith; Siebenwirth, Christian; Romer, Tina; Friedl, Anna A; Rothbauer, Ulrich
Abstract:
Poly(ADP-ribose) polymerase 1 (PARP1) is a key player in DNA repair, genomic stability and cell survival and it emerges as a highly relevant target for cancer therapies. To deepen our understanding of PARP biology and mechanisms of action of PARP1-targeting anti-cancer compounds, we generated a novel PARP1-affinity reagent, active both in vitro and in live cells. This PARP1-biosensor is based on a PARP1-specific single-domain antibody fragment (~ 15 kDa), termed nanobody, which recognizes the N-...     »
Journal title abbreviation:
PLoS ONE
Year:
2016
Journal volume:
11
Journal issue:
3
Pages contribution:
e0151041
Language:
eng
Fulltext / DOI:
doi:10.1371/journal.pone.0151041
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/26950694
Print-ISSN:
1932-6203
TUM Institution:
Klinik und Poliklinik für RadioOnkologie und Strahlentherapie
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