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Titel:

Flow cytometry-based TCR-ligand Koff -rate assay for fast avidity screening of even very small antigen-specific T cell populations ex vivo.

Dokumenttyp:
Journal Article
Autor(en):
Nauerth, Magdalena; Stemberger, Christian; Mohr, Fabian; Weißbrich, Bianca; Schiemann, Matthias; Germeroth, Lothar; Busch, Dirk H
Abstract:
High epitope-specific sensitivity of CD8(+) T cells is required for optimal immune protection against intracellular pathogens as well as certain malignancies. The quality of antigen recognition of CD8(+) T cells is usually described as "avidity" to its cognate peptide MHCI complex. T cell avidity is mainly dependent on the structural qualities of the T cell receptor (TCR), as convincingly demonstrated by recombinant TCR re-expression experiments. Based on reversible MHCI multimer staining and ko...     »
Zeitschriftentitel:
Cytometry A
Jahr:
2016
Band / Volume:
89
Heft / Issue:
9
Seitenangaben Beitrag:
816-25
Sprache:
eng
Volltext / DOI:
doi:10.1002/cyto.a.22933
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/27564267
Print-ISSN:
1552-4922
TUM Einrichtung:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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