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Titel:

T cell-specific inactivation of mouse CD2 by CRISPR/Cas9.

Dokumenttyp:
Journal Article
Autor(en):
Beil-Wagner, Jane; Dössinger, Georg; Schober, Kilian; Vom Berg, Johannes; Tresch, Achim; Grandl, Martina; Palle, Pushpalatha; Mair, Florian; Gerhard, Markus; Becher, Burkhard; Busch, Dirk H; Buch, Thorsten
Abstract:
The CRISPR/Cas9 system can be used to mutate target sequences by introduction of double-strand breaks followed by imprecise repair. To test its use for conditional gene editing we generated mice transgenic for CD4 promoter-driven Cas9 combined with guide RNA targeting CD2. We found that within CD4(+) and CD8(+) lymphocytes from lymph nodes and spleen 1% and 0.6% were not expressing CD2, respectively. T cells lacking CD2 carryied mutations, which confirmed that Cas9 driven by cell-type specific p...     »
Zeitschriftentitel:
Sci Rep
Jahr:
2016
Band / Volume:
6
Seitenangaben Beitrag:
21377
Sprache:
eng
Volltext / DOI:
doi:10.1038/srep21377
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/26903281
Print-ISSN:
2045-2322
TUM Einrichtung:
Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
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