Microbial colonization of pancreatic duct stents: a prospective analysis.

Schneider, Jochen; Schenk, Philipp; Obermeier, Andreas; Fremd, Julia; Feihl, Susanne; Forkl, Stefanie; Wantia, Nina; Römmler, Franziska; Neu, Bruno; Bajbouj, Monther; von Delius, Stefan; Schmid, Roland M; Algül, Hana; Weber, Andreas

doi:10.1097/MPA.0000000000000332

User: Guest

journal article

Title:: Microbial colonization of pancreatic duct stents: a prospective analysis.
Document type:: Journal Article
Author(s):: Schneider, Jochen; Schenk, Philipp; Obermeier, Andreas; Fremd, Julia; Feihl, Susanne; Forkl, Stefanie; Wantia, Nina; Römmler, Franziska; Neu, Bruno; Bajbouj, Monther; von Delius, Stefan; Schmid, Roland M; Algül, Hana; Weber, Andreas
Abstract:: The aim of the study was to analyze the microbial colonization rate as well as the spectrum and number of microorganisms in relation to the indwelling time of pancreatic stents.Forty pancreatic stents were prepared according to a standardized protocol and subsequently sonicated to optimize bacterial release from the biofilm on the stents.Two hundred forty-six microorganisms were identified. Thirty-nine of 40 stents were colonized with microorganisms. Aerobic gram-positive microorganisms (106/246 [43%]) accounted for the greatest proportion. The predominant microorganisms were Streptococcus species (46/246 [19%]), which were isolated from 27 (68%) of 40 stents. Stents with a short indwelling time (3-13 days) were mainly colonized with aerobic gram-positive bacteria (82%) and Candida species (63%). In contrast, anaerobes (P < 0.01, 69% vs 18%) and aerobic gram-negative microorganisms (P < 0.01, 93% vs 45%) such as Enterobacteriaceae (P < 0.01, 86% vs 27%) were significantly more present on stents with a long indwelling time (29-93 days), compared with stents with a short indwelling time.Microbial analysis of pancreatic duct stents revealed a very high colonization rate. Furthermore, the spectrum and number of microorganisms altered with the indwelling time of the stent. However, clinical relevance of our findings remains unclear.
Journal title abbreviation:: Pancreas
Year:: 2015
Journal volume:: 44
Journal issue:: 5
Pages contribution:: 786-90
Language:: eng
Fulltext / DOI:: doi:10.1097/MPA.0000000000000332
Pubmed ID:: http://view.ncbi.nlm.nih.gov/pubmed/25906452
Print-ISSN:: 0885-3177
TUM Institution:: II. Medizinische Klinik und Poliklinik (Gastroenterologie); Institut für Medizinische Mikrobiologie, Immunologie und Hygiene
BibTeX