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Title:

High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography.

Document type:
Journal Article; Research Support, Non-U.S. Gov't; Article
Author(s):
Stiel, Andre C; Deán-Ben, Xosé Luís; Jiang, Yuanyuan; Ntziachristos, Vasilis; Razansky, Daniel; Westmeyer, Gil G
Abstract:
Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins' contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic...     »
Journal title abbreviation:
Opt Lett
Year:
2015
Journal volume:
40
Journal issue:
3
Pages contribution:
367-70
Language:
eng
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/25680049
Print-ISSN:
0146-9592
TUM Institution:
Klinik und Poliklinik für Nuklearmedizin; Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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