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Title:

Neural labeling and manipulation by neonatal intraventricular viral injection in mice.

Document type:
Journal Article; Research Support, Non-U.S. Gov't
Author(s):
Wang, Mengzhe; Misgeld, Thomas; Brill, Monika S
Abstract:
This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other vir...     »
Journal title abbreviation:
STAR Protoc
Year:
2022
Journal volume:
3
Journal issue:
1
Fulltext / DOI:
doi:10.1016/j.xpro.2021.101081
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/35059654
TUM Institution:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
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