Benutzer: Gast  Login
Titel:

An optimized quantitative proteomics method establishes the cell type-resolved mouse brain secretome.

Dokumenttyp:
Article; Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Tüshaus, Johanna; Müller, Stephan A; Kataka, Evans Sioma; Zaucha, Jan; Sebastian Monasor, Laura; Su, Minhui; Güner, Gökhan; Jocher, Georg; Tahirovic, Sabina; Frishman, Dmitrij; Simons, Mikael; Lichtenthaler, Stefan F
Abstract:
To understand how cells communicate in the nervous system, it is essential to define their secretome, which is challenging for primary cells because of large cell numbers being required. Here, we miniaturized secretome analysis by developing the "high-performance secretome protein enrichment with click sugars" (hiSPECS) method. To demonstrate its broad utility, hiSPECS was used to identify the secretory response of brain slices upon LPS-induced neuroinflammation and to establish the cell type-re...     »
Zeitschriftentitel:
EMBO J
Jahr:
2020
Band / Volume:
39
Heft / Issue:
20
Volltext / DOI:
doi:10.15252/embj.2020105693
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/32954517
Print-ISSN:
0261-4189
TUM Einrichtung:
Lehrstuhl für Zellbiologie des Nervensystems (Prof. Misgeld)
 BibTeX