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Title:

Bacterial encapsulins as orthogonal compartments for mammalian cell engineering.

Document type:
Article
Author(s):
Sigmund, Felix; Massner, Christoph; Erdmann, Philipp; Stelzl, Anja; Rolbieski, Hannes; Desai, Mitul; Bricault, Sarah; Woerner, Tobias P.; Snijder, Joost; Geerlof, Arie; Fuchs, Helmut; de Angelis, Martin Hrabe; Heck, Albert J. R.; Jasanoff, Alan; Ntziachristos, Vasilis; Plitzko, Juergen; Westmeyer, Gil G.
Abstract:
We genetically controlled compartmentalization in eukaryotic cells by heterologous expression of bacterial encapsulin shell and cargo proteins to engineer enclosed enzymatic reactions and size-constrained metal biomineralization. The shell protein (EncA) from Myxococcus xanthus auto-assembles into nanocompartments inside mammalian cells to which sets of native (EncB,C,D) and engineered cargo proteins self-target enabling localized bimolecular fluorescence and enzyme complementation. Encapsulatio...     »
Journal title abbreviation:
Nat Commun
Year:
2018
Journal volume:
9
Journal issue:
1
Fulltext / DOI:
doi:10.1038/s41467-018-04227-3
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/29777103
Print-ISSN:
2041-1723
TUM Institution:
Klinik und Poliklinik für Nuklearmedizin; Lehrstuhl für Biologische Bildgebung - Zusammenarbeit mit dem Helmholtz-Zentrum München (Prof. Ntziachristos)
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