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Titel:

Characterization of signalling pathways by reverse phase protein arrays.

Dokumenttyp:
Journal Article
Autor(en):
Malinowsky, K; Wolff, C; Schott, C; Becker, KF
Abstract:
Reverse phase protein array (RPPA) is a very suitable technique to analyze large numbers of proteins in small samples like for example tumor biopsies. Beside their small size another major hindrance for the analysis of proteins from biopsies is the extraction of proteins from formalin-fixed and paraffin-embedded (FFPE) tissues. Here we describe a protocol, allowing quantitative extraction of large numbers of proteins from FFPE tissues and their subsequent analysis by RPPA. To elucidate the role...     »
Zeitschriftentitel:
Methods Mol Biol
Jahr:
2013
Band / Volume:
1049
Seitenangaben Beitrag:
285-99
Sprache:
eng
Volltext / DOI:
doi:10.1007/978-1-62703-547-7_21
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/23913224
Print-ISSN:
1064-3745
TUM Einrichtung:
Institut für Allgemeine Pathologie und Pathologische Anatomie
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