Follicular dendritic cells emerge from ubiquitous perivascular precursors.

The differentiation of follicular dendritic cells (FDC) is essential to the remarkable microanatomic plasticity of lymphoid follicles. Here we show that FDC arise from ubiquitous perivascular precursors (preFDC) expressing platelet-derived growth factor receptor ? (PDGFR?). PDGFR?-Cre-driven reporter gene recombination resulted in FDC labeling, whereas conditional ablation of PDGFR?(+)-derived cells abolished FDC, indicating that FDC originate from PDGFR?(+) cells. Lymphotoxin-?-overexpressing prion protein (PrP)(+) kidneys developed PrP(+) FDC after transplantation into PrP(-) mice, confirming that preFDC exist outside lymphoid organs. Adipose tissue-derived PDGFR?(+) stromal-vascular cells responded to FDC maturation factors and, when transplanted into lymphotoxin ? receptor (LT?R)(-) kidney capsules, differentiated into Mfge8(+)CD21/35(+)Fc?RII?(+)PrP(+) FDC capable of trapping immune complexes and recruiting B cells. Spleens of lymphocyte-deficient mice contained perivascular PDGFR?(+) FDC precursors whose expansion required both lymphoid tissue inducer (LTi) cells and lymphotoxin. The ubiquity of preFDC and their strategic location at blood vessels may explain the de novo generation of organized lymphoid tissue at sites of lymphocytic inflammation.     «

The differentiation of follicular dendritic cells (FDC) is essential to the remarkable microanatomic plasticity of lymphoid follicles. Here we show that FDC arise from ubiquitous perivascular precursors (preFDC) expressing platelet-derived growth factor receptor ? (PDGFR?). PDGFR?-Cre-driven reporter gene recombination resulted in FDC labeling, whereas conditional ablation of PDGFR?(+)-derived cells abolished FDC, indicating that FDC originate from PDGFR?(+) cells. Lymphotoxin-?-overexpressing p...     »