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Titel:

Tetracysteine-tagged prion protein allows discrimination between the native and converted forms.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't; Article
Autor(en):
Gaspersic, J; Hafner-Bratkovic, I; Stephan, M; Veranic, P; Bencina, M; Vorberg, I; Jerala, R
Abstract:
The conformational conversion of prion protein (PrP) from a native conformation to the amyloid form is a hallmark of transmissible spongiform encephalopathies. Conversion is usually monitored by fluorescent dyes, which bind generic amyloids and are less suited for living cell imaging. We report a new method for the synthesis of membrane-permeable and membrane-impermeable biarsenical reagents, which are then used to monitor murine PrP (mPrP) misfolding. We introduced tetracysteine (TC) tags into...     »
Zeitschriftentitel:
FEBS J
Jahr:
2010
Band / Volume:
277
Heft / Issue:
9
Seitenangaben Beitrag:
2038-50
Sprache:
eng
Volltext / DOI:
doi:10.1111/j.1742-4658.2010.07619.x
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/20345906
Print-ISSN:
1742-464X
TUM Einrichtung:
Institut für Virologie
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