A new quantitation method was developed for polyethylene glycol (PEG) homologues with various molecular weights. Separation of matrix compounds and analytes was achieved by solid phase extraction on C
18-cartridges. PEG oligomers were resolved by RP-HPLC coupled to mass spectrometry in multiple ion scan modus. [
13C
42H
4]- and [
13C
82H
8]-PEG homologues were synthesized and served as labelled internal standards for a stable isotope dilution assay. Urine samples from human and animal studies were analyzed with the validated method to characterize the permeability of the intestinal tract.
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A new quantitation method was developed for polyethylene glycol (PEG) homologues with various molecular weights. Separation of matrix compounds and analytes was achieved by solid phase extraction on C
18-cartridges. PEG oligomers were resolved by RP-HPLC coupled to mass spectrometry in multiple ion scan modus. [
13C
42H
4]- and [
13C
82H
8]-PEG homologues were synthesized and served as labelled internal standards for a stable isotope dilution assay. Urine samples from human and animal studies were anal...
»