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Original title:
Selektive ω-Oxidation aliphatischer Substrate – Charakterisierung von Monooxygenasen und Etablierung einer Biotransformationsplattform
Translated title:
Selective ω-oxidation of aliphatic substrates – Characterizing of monooxygenases and development of a biotransformation platform
Author:
Rimmel, Nina
Year:
2016
Document type:
Dissertation
Faculty/School:
Fakultät Wissenschaftszentrum Weihenstephan
Advisor:
Sieber, Volker (Prof. Dr.)
Referee:
Sieber, Volker (Prof. Dr.); Schwab, Wilfried (Prof. Dr.)
Language:
de
Subject group:
BIO Biowissenschaften
Keywords:
Biotransformation, Cytochrom P450, Candida tropicalis, Downscale, ω-Oxidation
Translated keywords:
Biotransformation, Cytochrome P450, Candida tropicalis, downscale, ω-Oxidation
TUM classification:
CIT 680d
Abstract:
Zur Untersuchung und Optimierung einer industriellen Oxidation von Fettsäuren und deren Methylestern durch Candida tropicalis wurde in einem Downscale-Ansatz eine Biotransformationsplattform etabliert. Neben der Untersuchung von Parametern, wie Temperatur und Substratfeedstrategie, wurden zur Entwicklung zukünftiger C. tropicalis Produktionsstämme verschiedene neue Cytochrom P450 Enzyme identifiziert und bezüglich ihres Substrat- und Produktspektrums charakterisiert.
Translated abstract:
To get deeper insights into an industrial oxidation of fatty acids and their corresponding methyl esters, a down scaled biotransformation platform was established. Parameter like temperature levels, substrate feeding and effects on the biocatalyst Candida tropicalis were studied. For the future development of new production strains, various Cytochrome P450 enzymes were identified and characterized, regarding their substrate and product spectra.
WWW:
https://mediatum.ub.tum.de/?id=1280105
Date of submission:
17.02.2016
Oral examination:
21.06.2016
File size:
4647749 bytes
Pages:
159
Urn (citeable URL):
https://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:91-diss-20160621-1280105-1-3
Last change:
21.11.2016
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