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Title:

POSH stimulates the ubiquitination and the clathrin-independent endocytosis of ROMK1 channels.

Document type:
Journal Article; Research Support, N.I.H., Extramural; Article
Author(s):
Lin, DH; Yue, P; Pan, CY; Sun, P; Zhang, X; Han, Z; Roos, M; Caplan, M; Giebisch, G; Wang, WH
Abstract:
POSH (plenty of SH3) is a scaffold protein that has been shown to act as an E3 ubiquitin ligase. Here we report that POSH stimulates the ubiquitination of Kir1.1 (ROMK) and enhances the internalization of this potassium channel. Immunostaining reveals the expression of POSH in the renal cortical collecting duct. Immunoprecipitation of renal tissue lysate with ROMK antibody and glutathione S-transferase pulldown experiments demonstrated the association between ROMK and POSH. Moreover, immunopreci...     »
Journal title abbreviation:
J Biol Chem
Year:
2009
Journal volume:
284
Journal issue:
43
Pages contribution:
29614-24
Language:
eng
Fulltext / DOI:
doi:10.1074/jbc.M109.041582
Pubmed ID:
http://view.ncbi.nlm.nih.gov/pubmed/19710010
Print-ISSN:
0021-9258
TUM Institution:
Fachgebiet Nephrologie (Prof. Heemann)
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