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- Title:
The role of the Bcl-3 proto-oncogene in thyroid hormone-induced liver cell proliferation.
- Document type:
- Journal Article; Research Support, Non-U.S. Gov't
- Author(s):
- Malik, R; Bungay, A; Sadiq, A; Brown, D; Riemann, M; Choudhury, S; Schmid, R; Selden, C; Hodgson, H
- Abstract:
- The aim of the study was to determine if thyroid hormone-induced liver cell proliferation occurs through the Bcl-3 proto-oncogene. Rodents (including Bcl-3 knockout mice and the wild-type strain) were injected with a single dose of tri-iodothyronine (T(3)) and sacrificed at various time points. Hepatic mRNA (real-time polymerase chain reaction ) and protein expression (Western analysis) of Bcl-3 was quantified in rats stimulated with T(3). Cell proliferation was induced in a variety of cell types after T(3) injection at 24 h including hepatocytes (7 +/- 1.1% vs. 0.45 +/- 0.025%; P < 0.01), hepatic nonparenchymal cells (3.8 +/- 1.2% vs. 0.3 +/- 0.01%; P < 0.01), renal tubular cells (8.1 +/- 1.6% vs. 0.2 +/- 0.035%; P < 0.01), and splenic lymphocytes (4.8 +/- 1.2% vs. 0.35 +/- 0.02%; P < 0.01). We showed a twofold increase in hepatic Bcl-3 mRNA (P < 0.01) and protein expression (P < 0.01) at 24 h in rats stimulated with T(3). However, there were no differences in the rate of liver cell proliferation between Bcl-3 knockout mice and the wild-type strain (0.4 +/- 0.15% vs. 0.3 +/- 0.1%), indicating that Bcl-3 was not functionally involved in thyroid hormone-induced liver cell proliferation. A single gene is unlikely to initiate the process of thyroid hormone-induced cell proliferation. A complex interaction between the genomic and nongenomic effects of thyroid hormone is likely to regulate the mitogenic effects.
- Journal title abbreviation:
- Artif Organs
- Year:
- 2009
- Journal volume:
- 33
- Journal issue:
- 6
- Pages contribution:
- 425-30
- Language:
- eng
- Fulltext / DOI:
- doi:10.1111/j.1525-1594.2009.00752.x
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/19473137
- Print-ISSN:
- 0160-564X
- TUM Institution:
- II. Medizinische Klinik und Poliklinik (Gastroenterologie)
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