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- Title:
The effect of selective inhibition of inducible nitric oxide synthase on cytochrome P450 after liver transplantation in a rat model.
- Document type:
- Journal Article; Proceedings Paper
- Author(s):
- Matevossian, E; Novotny, A; Knebel, C; Brill, T; Werner, M; Sinicina, I; Kriner, M; Stangl, M; Thorban, S; Hüser, N
- Abstract:
- BACKGROUND: Activity levels of cytochrome P450 (CYP) provide markers for liver function and graft rejection episodes after orthotopic liver transplantation (OLT). Some in vitro studies have shown decreased CYP activation of inducible nitric oxide synthase (iNOS) in rejecting liver grafts. The aim of this study was to evaluate CYP isoenzyme activity changes in vivo and to examine histopathologic aspects during inhibition of iNOS after treatment with aminoguanidine (AG) using OLT in the rat. MATERIALS AND METHODS: Thirty DA-(RT1av1) rats that served as donors and LEWIS-(RT(1)) rats as recipients were divided into three groups: group I (controls, syngeneic rats; n = 6), group II (allogeneic rats without immunosupression; n = 11), and group III (allogeneic rats with AG treatment; n = 13). On postoperative days 5, 8, and 10 we performed laboratory investigations and liver biopsies for histopathologic investigations. RESULTS: On postoperative day 5, activities of CYP-1A1 and -3A4 were significantly lower (P = .022) in group III and the activity of CYP-1A2 higher (P < .05) compared with group II. At postoperative days 8 and 10, the activities of all CYP isoenzymes were significant higher in AG-treated rats (group III) in contrast with group II after allogeneic OLT without immunosuppression. Histopathologic findings revealed less distinct rejection signs in group III specimens after AG treatment compared with group II. CONCLUSION: Summarizing our results, we concluded that AG treatment led to increased CYP activity and less distinction of graft rejection after OLT in rats.
- Journal title abbreviation:
- Transplant Proc
- Year:
- 2008
- Journal volume:
- 40
- Journal issue:
- 4
- Pages contribution:
- 983-5
- Language:
- eng
- Fulltext / DOI:
- doi:10.1016/j.transproceed.2008.04.001
- Pubmed ID:
- http://view.ncbi.nlm.nih.gov/pubmed/18555096
- Print-ISSN:
- 0041-1345
- TUM Institution:
- Chirurgische Klinik und Poliklinik; Institut für Experimentelle Onkologie und Therapieforschung; Institut für Medizinische Statistik und Epidemiologie
- Format:
- Text
BibTeX
Occurrences:
- mediaTUM GesamtbestandEinrichtungenSchools TUM School of Medicine and HealthEhemalige EinrichtungenInstitut für Experimentelle Onkologie und Therapieforschung2008
- mediaTUM GesamtbestandHochschulbibliographie2008FakultätenMedizinInstitut für Medizinische Statistik und Epidemiologie
- mediaTUM GesamtbestandHochschulbibliographie2008FakultätenMedizinInstitut für Experimentelle Onkologie und Therapieforschung