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Original title:
Development of a loop-mediated isothermal amplification assay for the detection of the pear decline agent Candidatus Phytoplasma pyri 
Translated title:
Entwicklung eines Loop-mediated isothermal amplification-Assays für den Nachweis des Birnenverfall-Erregers Candidatus Phytoplasma pyri 
Year:
2019 
Document type:
Dissertation 
Institution:
Fakultät Wissenschaftszentrum Weihenstephan 
Advisor:
Schwab, Wilfried (Prof. Dr.) 
Referee:
Schwab, Wilfried (Prof. Dr.); Niessen, Ludwig (Prof. Dr.) 
Language:
en 
Subject group:
LAN Landbauwissenschaft 
Keywords:
Loop-mediated isothermal amplification, pear decline, Candidatus Phytoplasma pyri, eriochromeblack-T 
Translated keywords:
Loop-mediated isothermal amplification, Birnenverfall, Candidatus Phytoplasma pyri, Eriochromschwarz-T 
TUM classification:
LAN 410d 
Abstract:
Pear decline (PD) is a quarantine disease causing reduced yield, stunting and dieback of pears in all pear-growing countries. The objective of this work was the development of a loop-mediated isothermal amplification (LAMP) procedure for the detection of Candidatus Phytoplasma pyri, the causal agent of PD. Primers targeting the 16S rRNA gene of the pathogen were designed and LAMP reaction conditions were optimized for colorimetric detection with the metal indicator Eriochromeblack-T. A sample pr...    »
 
Translated abstract:
Birnenverfall (PD) ist eine Quarantänekrankheit, die verringerte Erträge, Kümmerwuchs und Absterben von Birnbäumen in allen Birnenanbaugebieten der Welt verursacht. Ziel dieser Arbeit war die Entwicklung einer Loop-mediated isothermal amplification-Prozedur (LAMP) für den Nachweis von Candidatus Phytoplasma pyri, dem Erreger von PD. Primer zur Amplifizierung des 16S rRNA-Gens des Erregers wurden designed und die LAMP-Reaktionsbedingungen für colorimetrische Detektion mithilfe des Metallindikator...    »
 
Oral examination:
07.02.2019 
File size:
5625646 bytes 
Pages:
163 
Last change:
15.03.2019