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Title:

TMT Labeling for the Masses: A Robust and Cost-efficient, In-solution Labeling Approach

Document type:
Zeitschriftenaufsatz
Author(s):
Zecha, Jana; Satpathy, Shankha; Kanashova, Tamara; Avanessian, Shayan C.; Kane, M. Harry; Clauser, Karl R.; Mertins, Philipp; Carr, Steven A.; Kuster, Bernhard
Abstract:
Isobaric stable isotope labeling using, for example, tandem mass tags (TMTs) is increasingly being applied for large-scale proteomic studies. Experiments focusing on proteoform analysis in drug time course or perturbation studies or in large patient cohorts greatly benefit from the reproducible quantification of single peptides across samples. However, such studies often require labeling of hundreds of micrograms of peptides such that the cost for labeling reagents represents a major contributio...     »
Keywords:
BayBioMS; Tandem Mass Tags; Stable isotope labeling; Labeling efficiency; NHS ester chemistry; Peptides*; Phosphoproteome; Post-translational modifications*; Quantification; Tandem Mass Spectrometry
Journal title:
Molecular & Cellular Proteomics
Year:
2019
Journal volume:
18
Journal issue:
7
Pages contribution:
1468-1478
Fulltext / DOI:
doi:10.1074/mcp.tir119.001385
Publisher:
American Society for Biochemistry & Molecular Biology (ASBMB)
E-ISSN:
1535-94761535-9484
Date of publication:
09.04.2019
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