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Titel:

Hepatitis B Virus Targets Lipid Transport Pathways to Infect Hepatocytes.

Dokumenttyp:
Journal Article; Research Support, Non-U.S. Gov't
Autor(en):
Esser, Knud; Cheng, Xiaoming; Wettengel, Jochen M; Lucifora, Julie; Hansen-Palmus, Lea; Austen, Katharina; Roca Suarez, Armando A; Heintz, Sarah; Testoni, Barbara; Nebioglu, Firat; Pham, Minh Tu; Yang, Shangqing; Zernecke, Alma; Wohlleber, Dirk; Ringelhan, Marc; Broxtermann, Mathias; Hartmann, Daniel; Hüser, Norbert; Mergner, Julia; Pichlmair, Andreas; Thasler, Wolfgang E; Heikenwalder, Mathias; Gasteiger, Georg; Blutke, Andreas; Walch, Axel; Knolle, Percy A; Bartenschlager, Ralf; Protzer, Ulrik...     »
Abstract:
BACKGROUND & AIMS: A single hepatitis B virus (HBV) particle is sufficient to establish chronic infection of the liver after intravenous injection, suggesting that the virus targets hepatocytes via a highly efficient transport pathway. We therefore investigated whether HBV uses a physiological liver-directed pathway that supports specific host-cell targeting in vivo. METHODS: We established the ex vivo perfusion of intact human liver tissue that recapitulates the liver physiology to investigate HBV liver targeting. This model allowed us to investigate virus-host cell interactions in a cellular microenvironment mimicking the in vivo situation. RESULTS: HBV was rapidly sequestered by liver macrophages within 1 hour after a virus pulse perfusion but was detected in hepatocytes only after 16 hours. We found that HBV associates with lipoproteins in serum and within machrophages. Electron and immunofluorescence microscopy corroborated a co-localization in recycling endosomes within peripheral and liver macrophages. Recycling endosomes accumulated HBV and cholesterol, followed by transport of HBV back to the cell surface along the cholesterol efflux pathway. To reach hepatocytes as final target cells, HBV was able to utilize the hepatocyte-directed cholesterol transport machinery of macrophages. CONCLUSIONS: Our results propose that by binding to liver targeted lipoproteins and using the reverse cholesterol transport pathway of macrophages, HBV hijacks the physiological lipid transport pathways to the liver to most efficiently reach its target organ. This may involve transinfection of liver macrophages and result in deposition of HBV in the perisinusoidal space from where HBV can bind its receptor on hepatocytes.
Zeitschriftentitel:
Cell Mol Gastroenterol Hepatol
Jahr:
2023
Band / Volume:
16
Heft / Issue:
2
Seitenangaben Beitrag:
201-221
Volltext / DOI:
doi:10.1016/j.jcmgh.2023.03.011
PubMed:
http://view.ncbi.nlm.nih.gov/pubmed/37054914
TUM Einrichtung:
Institut für Experimentelle Onkologie und Therapieforschung; Klinik und Poliklinik für Chirurgie (Prof. Friess); Klinik und Poliklinik für Innere Medizin II, Gastroenterologie (Prof. Schmid); Roman Herzog Comprehensive Cancer Center
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